13 August, 2001
Fun With Bacteria Monday, 13 August 2001
Hej! Hur star det till? (Hello! How are you?)
Life on Board
Whenever we have a really cold day, on ropes and rough surfaces all over the ship a special kind of ice forms. Rime ice can take many forms and shapes, depending on the temperature of the air and the surface it forms on. The most spectacular form is long spicules of ice crystals. Some of these form when small drops of water fall onto the surface then freeze on the outside, forming a small shell of ice with water inside. Pressure inside this little package builds then bursts open the shell, spitting out a smaller drop of water that spatters around the outside then freezes. The drop freezes up again then bursts open again, spitting out an even smaller drop that also freezes, and so on. This results in a sort of fireworks-looking rime ice crystal. There is a whole science behind these crystal formations but regardless of that, the outcome is a beautiful work of nature's art.
Where Are We Now?
It was a cloudy day with light snow most of the day. Our coordinates at 2 pm were 88o25' North by 1o36' West, so we are still drifting west.
Scientists at Work
Whenever we take a microlayer surface sample, it must be processed for bacteria. Johan Knulst, from IVL, Aneboda Research Facility in Sweden, collects these microlayer samples from open water leads that form near the ship whenever it is not too windy for his little sampler boats (everybody volunteers to help him because they like to drive these little radio-controlled boats, especially the guys on board). Surface layer samples are collected in bottles on the boats, picked up by a specially-coated rolling drum at the front of the boat. Whenever you see a smooth smear (a slick) on the surface of a body of water, it is probably the result of a concentration of organic compounds and microorganisms, which tend to stick together. You can feel the difference between his microlayer samples and pure water. The microlayer sample feels more viscous and oily than water, even though these in the Arctic are naturally-produced organic compounds. Back in the lab, he splits the sample into very small 2-mL vials, then adds a specific amount of a basic protein called leucine to each one. Johan wants to see how active the bacteria are so the vials go into the freezing incubator for 24 hours, giving the live bacteria a chance to gobble up this protein snack. What the bacteria don't know is that this is special leucine that contains tritium, a low-radiation isotope of hydrogen, which can be later counted by an instrument called a Liquid Scintillation Counter.
After 24 hours, we bring the vials into the lab and "fix" them (that is a biologist's way of saying kill them) with another compound called trichloro acetic acid, or TCA. Then, we put the vials into a centrifuge and spin them really fast, concentrating the bacteria on one side of the vial. Next, without disturbing this bacteria "pellet," we suck out the leftover leucine and seawater using what Johan calls the "slurpee," a suction device that sounds like that thing the dentist puts in your mouth. Then we wash and centrifuge and slurpee the bacteria twice more and finally add some other stuff called a scintillation cocktail (yummy!) that allows the radioactive isotope to be counted by the instrument. As a matter of fact, it is 9 pm and yesterday's bacteria are ready to have a ride on the merry-go-round. Off to the circus I go!
Vi ses! (See you later!)
From Deck 4 on the Icebreaker Oden, drifting west, north of 88, Dena Rosenberger
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